Add 2 ml of diluted wash buffer to each well.Overnight Assay ( long procedure) Rapid Assay Procedure
WESTERN BLOT HIV MANUAL
Test procedure according to the manual of MP Diagnostics for HIV BLOT 2.2
It can be further completed by either chromogenic detection or. Protein transfer to a membrane: membrane stain and blocking and washing. Gel electrophoresis of protein: It may be performed by gel stain or. Sample preparation ( protein extraction). Paper towel, adhesive tape, worksheet (non-absorbent white paper). Sodium hypochlorite for decontamination. Aspirator with sodium hypochlorite trap. Precision Pipettes ranging from 20µl to 1000µl dispensing volume. Rocking platform (designed with a rocking speed range of 12 to 16Ĭycles per minute, and which moves through a 5° to 10° tilt to wash. Incorporated with HIV-1 viral lysate, a. The sodium dodecyl sulfate (SDS) Polyacrylamide gel electrophoresis (PAGE) technique is a prerequisite for Western blotting. A technique for detecting specific proteins separated by electrophoresis by the use of labeled antibodies. In Western blotting, the molecule of interest is a protein and the probe is typically an antibody raised against that particular protein. It is an Immunoblotting technique that relies on the specificity of binding between a molecule of interest and a probe to allow detection of the molecule of interest in a mixture of many other similar molecules. Western blot: It is applicable for transferring protein andĮastern blot: It is a biochemical technique used to analyze protein post-translational modifications (PTM) and is most often used to detect carbohydrate epitope. 
Northern blot: It uses for transferring RNA. Southern blot: It uses for transferring DNA. Blot is a technique for transferring DNA, RNA, and proteins onto a carrier so they can be separated, and often follows the use of gel electrophoresis. It is also called immunoblotting and it is a technique for detecting specific proteins separated by electrophoresis by the use of labeled antibodies. Western blot test is a confirmatory test of HIV-AIDS and it is positive as shown above image.
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